Part:BBa_I721007:Design

pAraC/BAD>ERBS>LacI>ERBS>TetR>ERBS>mRFP1>Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2383
  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  Illegal AgeI site found at 3681
  Illegal AgeI site found at 3793
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

Designed to repress the other 2 pathways involved in the Tristable Switch. Also designed to indicate when it is being expressed.

Source

This is a composite part formed from basic iGem parts.